Poly-L-lysine amplification of protamine immobilization and heparin adsorption
نویسندگان
چکیده
منابع مشابه
Poly-L-lysine amplification of protamine immobilization and heparin adsorption.
We previously reported the development of a cellulose fiber based blood filter device containing immobilized protamine (termed protamine filter) that could be used to control both heparin- and protamine-induced complications during extracorporeal therapy. To achieve enhanced heparin adsorption on the fibers, we examined the possibility of utilizing the poly-L-lysine based amplification method t...
متن کاملProtamine immobilization and heparin adsorption on the protamine-bound cellulose fiber membrane.
Immobilization of protamine to the inner lumen of cellulose hollow fibers has been shown useful in preventing both heparin- and protamine-induced complications during an extracorporeal blood circulation procedure. The current study examined the effects of variables on the immobilization of protamine to cyanogen bromide (CNBr)-activated cellulose hollow fibers. The degree of protamine immobiliza...
متن کاملEnhanced epsilon-poly-L-lysine production from Streptomyces ahygroscopicus by a combination of cell immobilization and in situ adsorption.
Epsilon-poly-L-lysine (epsilon-PL), produced by Streptomyces or Kitasatospora strains, is a homo-poly-amino acid of Llysine, which is used as a safe food preservative. The present study investigates the combined use of cell immobilization and in situ adsorption (ISA) to produce epsilon-PL in shaken flasks. Loofah sponge-immobilized Streptomyces ahygroscopicus GIM8 produced slightly more epsilon...
متن کاملAnti-angiogenic poly-L-lysine dendrimer binds heparin and neutralizes its activity.
The interaction between heparin, a polyanion, and a polycationic dendrimer with a glycine core and lysine branches Gly-Lys63(NH2)64 has been investigated. Complexation was assessed by transmission electron microscopy, size and zeta potential measurements, methylene blue spectroscopy, and measuring the anti-coagulant activity of heparin in vitro and in vivo. Complete association between the hepa...
متن کاملControl of the Specific Adsorption of Proteins onto Gold Surfaces with Poly( L-lysine) Monolayers
Monolayers of the polypeptide poly(L-lysine) (PL) are used to control the specific adsorption of proteins onto gold surfaces. A PL monolayer modified with biotin is electrostatically adsorbed onto a vapor-deposited gold Eilm that has been coated with a self-assembled monolayer of the alkanethiol 1 1-mercaptoundecanoic acid (MUA). The immobilized biotin moieties act as specific adsorption sites ...
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ژورنال
عنوان ژورنال: Journal of Biomedical Materials Research
سال: 1998
ISSN: 0021-9304,1097-4636
DOI: 10.1002/(sici)1097-4636(199811)42:2<182::aid-jbm3>3.0.co;2-l